CD117/c-kit Antibody (ACK2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43359
![Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-ACK2-Immunohistochemistry-Whole-Mount-NBP1-43359-img0003.jpg "Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Guinea Pig
Cited:
Human, Mouse, Rat, Guinea Pig
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # ACK2
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Mouse CD117
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 23967247). Guinea Pig reactivity reported in scientific literature (PMID: 21654812)
Specificity
The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor.
Marker
Hematopoietic Stem Cell Marker
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images for CD117/c-kit Antibody (ACK2) - BSA Free
Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]
Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (https://www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.![Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0001.jpg "Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]")
Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]
Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c![Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0002.jpg "Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]")
Flow Cytometry: CD117/c-kit Antibody (ACK2) - BSA Free [NBP1-43359]
Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells wereApplications for CD117/c-kit Antibody (ACK2) - BSA Free
Application
Recommended Usage
Flow Cytometry
< 0.125 ug/10^6 cells
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 26680736). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24161026).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NBP1-43359 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Entrez Gene IDs
3815 (Human)
Gene Symbol
KIT
UniProt
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (ACK2) - BSA Free
Product Specific Notices for CD117/c-kit Antibody (ACK2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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