CD117/c-kit Antibody (KIT/983)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44762
![Western Blot: CD117/c-kit Antibody (KIT/983) [NBP2-44762]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-KIT-983-Western-Blot-NBP2-44762-img0002.jpg "Western Blot: CD117/c-kit Antibody (KIT/983) [NBP2-44762]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # KIT/983
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa100-300) of human CD117/c-kit protein (exact sequence is proprietary) (Uniprot: P10721)
Localization
Cell surface and cytoplasmic
Specificity
This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposis sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Marker
Marker for Gastrointestinal Stromal Tumors
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11476)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD117/c-kit Antibody (KIT/983)
Western Blot: CD117/c-kit Antibody (KIT/983) [NBP2-44762]
Western Blot: CD117/c-kit Antibody (KIT/983) [NBP2-44762] - Western Blot analysis of recombinant fragment of CD117 using CD117/c-kit Antibody (KIT/983).Applications for CD117/c-kit Antibody (KIT/983)
Application
Recommended Usage
Western Blot
2-4 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
UniProt
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (KIT/983)
Product Specific Notices for CD117/c-kit Antibody (KIT/983)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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