CD117/c-kit Antibody (rC117/9253)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23868

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2b Kappa Clone # rC117/9253

Concentration

0.2 mg/ml

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD117/c-kit protein

Localization

Cell surface. Cytoplasm.

Specificity

This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Marker

Marker for Gastrointestinal Stromal Tumors

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for CD117/c-kit Antibody (rC117/9253)

CD117/c-kit Antibody (rC117/9253) Immunohistochemistry-Paraffin: CD117/c-kit Antibody (rC117/9253) [NBP3-23868] -

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (rC117/9253) [NBP3-23868] -

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (rC117/9253) [NBP3-23868] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Antibody (rC117/9253). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for CD117/c-kit Antibody (rC117/9253)

Application
Recommended Usage

ELISA

2-4ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
ELISA: For coating, order antibody without BSA.

Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (rC117/9253)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD117/c-kit Antibody (rC117/9253)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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