CD11b Antibody [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-89474PECY55
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque
Applications
Dual RNAscope ISH-IHC, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In-situ Hybridization, Simple Western, Single Cell Western, Western Blot
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD11b Antibody [PE/Cy5.5]
Immunogen
Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:35398596).Monkey reactivity reported in scientific literature (PMID: 26443820). Rhesus Macaque reactivity reported in scientific literature (PMID: 29760177). Use in C. elegans reported in scientific literature (PMID:32058942).
Localization
Monocytes and granulocytes.
Marker
Microglia Marker, Myeloid Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD11b Antibody [PE/Cy5.5]
Product Image: CD11b Antibody [PE/Cy5.5] [NB110-89474PECY55] - Vial of PE/Cy5.5 conjugated antibody. PE/Cy5.5 has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 566 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 700 nm.
Applications for CD11b Antibody [PE/Cy5.5]
Application
Recommended Usage
Dual RNAscope ISH-IHC
Optimal dilutions of this antibody should be experimentally determined.
Flow (Cell Surface)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
In-situ Hybridization
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Single Cell Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD11b/Integrin alpha M
References
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for CD11b Antibody [PE/Cy5.5]
Product Specific Notices for CD11b Antibody [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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