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CD11b/c Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40766V

Novus Biologicals, part of Bio-Techne
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NB110-40766V
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD11b/c Antibody [DyLight 405]

Immunogen

A synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25150592)

Localization

Monocytes and granulocytes.

Marker

Microglia Marker, Macrophage Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for CD11b/c Antibody [DyLight 405]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD11b/c

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

Alternate Names

antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide), Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement component 3 receptor 3 subunit), Leukocyte adhesion receptor MO1, MAC-1, MAC1A, macrophage antigen alpha polypeptide, MO1A, Neutrophil adherence receptor, neutrophil adherence receptor alpha-M subunit, SLEB6

Gene Symbol

ITGAM

Additional CD11b/c Products

Product Documents for CD11b/c Antibody [DyLight 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD11b/c Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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