Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # AP-MAB0806

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD11c Antibodies »

Product Specifications

Immunogen

This CD11c antibody was produced from a hybridoma (mouse myeloma fused with spleen cells) from an Armenian Hamster immunized with mouse lymphocytes.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30455684).

Marker

Dendritic Cell Marker

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for CD11c Antibody (AP-MAB0806) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) - Azide and BSA Free [NB110-97871]

Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) - Azide and BSA Free [NB110-97871]

Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Mouse spleen tissue.
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) - Azide and BSA Free [NB110-97871]

Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) - Azide and BSA Free [NB110-97871]

Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Negative control, mouse spleen tissue section.

Applications for CD11c Antibody (AP-MAB0806) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100-1:1000

Immunohistochemistry

1:25-1:100

Immunohistochemistry-Paraffin

1:25-1:100

Immunoprecipitation

1:100
Application Notes
4% PFA fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse CD11c. The antigen retrieval recommended for this antibody is the treatment with 20ug/ml Proteinase K in PBS for 15-25 minutes at room temperature. This antibody is CyTOF ready. Use in ICC/IF reported in scientific literature (PMID: 30300579). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31138656).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD11c

CD11c (integrin alpha X chain protein) combines with the beta 2 chain (ITGB2) to form a leukocyte specific integrin referred to as inactivated C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. The CD11c antigen is a marker for hairy cell leukemia.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Entrez Gene IDs

16411 (Mouse)

Gene Symbol

ITGAX

UniProt

Q9QXH4

Additional CD11c Products

Product Documents for CD11c Antibody (AP-MAB0806) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD11c Antibody (AP-MAB0806) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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