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CD11c Antibody (BU15) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45015

Clone BU15 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-45015
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BU15

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD11c Antibody (BU15) [FITC]

Immunogen

Dendritic cells of synovial fluid

Specificity

The antibody BU15 reacts with CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages. HLDA III; WS Code M 256 HLDA V; WS Code AS S143 HLDA VI; WS Code AS Ref.6

Marker

Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Scientific Data Images for CD11c Antibody (BU15) [FITC]

Flow Cytometry: CD11c Antibody (BU15) [FITC] [NBP1-45015] - Separation of human monocytes (red-filled) from CD11c negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11c (BU15) FITC (20 ul reagent / 100 ul of peripheral whole blood).
Flow Cytometry: CD11c Antibody (BU15) [FITC] [NBP1-45015] - Surface staining pattern of human peripheral whole blood stained using anti-human CD11c (BU15) FITC (20 ul reagent / 100 ul of peripheral whole blood).

Applications for CD11c Antibody (BU15) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD11c

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha-X. CD11c non-covalently associates with beta-2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

Additional CD11c Products

Product Documents for CD11c Antibody (BU15) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD11c Antibody (BU15) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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