CD11c Antibody (N418) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81066
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD11c Antibody (N418) - Chimeric - Azide and BSA Free [NBP2-81066]](https://resources.bio-techne.com/images/products/CD11c-Antibody-N418-Immunocytochemistry-Immunofluorescence-NBP2-81066-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD11c Antibody (N418) - Chimeric - Azide and BSA Free [NBP2-81066]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # N418
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Hamster spleen cells that had been primed to mouse spleen dendritic cells
Specificity
The antibody binds to mouse and human CD11c.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD11c Antibody (N418) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD11c Antibody (N418) - Chimeric - Azide and BSA Free [NBP2-81066]
Immunocytochemistry/Immunofluorescence: CD11c Antibody (N418) [NBP2-81066] - Immunofluorescence staining of fixed U937 cells with anti-CD11c antibody N418 [NBP2-81066]. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of N418 [NBP2-81066] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81066, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.Applications for CD11c Antibody (N418) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human CD11, which is part of the human integrin receptor for fibrinogen, recognizing a GPR motif. The receptor is involved in cell-cell contacts, chemotaxis, and inflammatory responses.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD11c
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Gene Symbol
ITGAX
Additional CD11c Products
Product Documents for CD11c Antibody (N418) - Chimeric - Azide and BSA Free
Product Specific Notices for CD11c Antibody (N418) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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