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CD14 Antibody (61D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28436

Clone 61D3 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Canine, Monkey

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 61D3

Concentration

0.1 mg/ml

Product Specifications

Immunogen

Human peripheral monocytes

Reactivity Notes

Hooded Seal CD14.

Specificity

Human/Cynomolgus/Canine/Hooded Seal CD14

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD14 Antibody (61D3)

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436] - Hooded seal bronchoalveolar lavage cells were stained with Mouse Anti-Human CD14-UNLB followed by a secondary antibody and DRAQ5. Image from Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186.
Flow Cytometry: CD14 Antibody (61D3) [NBP1-28436]

Flow Cytometry: CD14 Antibody (61D3) [NBP1-28436]

Flow Cytometry: CD14 Antibody (61D3) [NBP1-28436] - Analysis using the Allophycocyanin conjugate of NBP1-28436. Multiple staining of peripheral blood lymphocytes.

Applications for CD14 Antibody (61D3)

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD14

CD14 is a 53-55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. (1-4)

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (61D3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD14 Antibody (61D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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