CD14 Antibody (Sa14-2) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51121

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-51121
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # Sa14-2

Format

Azide Free

Concentration

0.1 mg/ml

View all CD14 Antibodies »

Product Specifications

Immunogen

The monoclonal antibody Sa14-2 reacts with the mouse monocyte marker CD14, a 53 kDa glycoprotein.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD14 Antibody (Sa14-2) - Azide Free

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (Sa14-2) [NBP1-51121]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (Sa14-2) [NBP1-51121]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (Sa14-2) [NBP1-51121] - RAW264.7 cells were grown on coverslips, fixed with 1% parformaldehyde and blocked with BSA. As primary antibody was used at 2 ug/ml in PBS/BSA 3%.
Flow Cytometry: CD14 Antibody (Sa14-2) [NBP1-51121]

Flow Cytometry: CD14 Antibody (Sa14-2) [NBP1-51121]

Flow Cytometry: CD14 Antibody (Sa14-2) [NBP1-51121] - 100000 RAW264.7 cells were stained with 1ug/ml antibody for 1h at 4C (black - no staining; Red - isotype c ontrol; blue-NBP1-51121)

Applications for CD14 Antibody (Sa14-2) - Azide Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:50
Application Notes
Positive control: RAW264.7 cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD14

The monoclonal antibody Sa14-2 reacts with the mouse monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present in low density on a subpopulation of granulocytes and activated or transformed B-cells.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (Sa14-2) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD14 Antibody (Sa14-2) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.