CD14 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2807

Novus Biologicals, part of Bio-Techne
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NB100-2807
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CD14 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRVDADADPRQYAD corresponding to internal region according to NP_000582.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CD14 Antibody

Western Blot: CD14 Antibody [NB100-2807]

Western Blot: CD14 Antibody [NB100-2807]

Western Blot: CD14 Antibody [NB100-2807] - Staining (1ug/ml) of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.
Flow Cytometry: CD14 Antibody [NB100-2807]

Flow Cytometry: CD14 Antibody [NB100-2807]

Flow Cytometry: CD14 Antibody [NB100-2807] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
CD14 Antibody

Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] -

Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] - (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Applications for CD14 Antibody

Application
Recommended Usage

ELISA

Detection limit 1:128000

Flow Cytometry

10 ug/ml

Immunohistochemistry

6-8 ug/ml

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx 55kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to the glycosylated form. Primary incubation 1 hour at room temperature. ICC/IF reactivity reported in (PMID: 25780044).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a single copy gene encoding 2 protein forms a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Alternate Names

CD14

Entrez Gene IDs

929 (Human)

Gene Symbol

CD14

UniProt

P08571

Additional CD14 Products

Product Documents for CD14 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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