CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12070
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free [NBP3-12070]](https://resources.bio-techne.com/images/products/CD15-Lewis-X-Antibody-BRA-4F1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12070-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free [NBP3-12070]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # BRA-4F1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free
Immunogen
This antibody was raised by immunzing mice with human CD15/Lewis X.
Specificity
This antibody is specific for CD15/Lewis X. It also shows crossreactivity with the CD15/Lewis X antigen from other species.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free [NBP3-12070]
Immunocytochemistry/Immunofluorescence: CD15/Lewis X Antibody (BRA-4F1) - Chimeric [NBP3-12070] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BRA-4F1 (NBP3-12070) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12070, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD15/Lewis X
SSEA1 antibodies are useful tools for differentiation studies and FACS analysis.
Alternate Names
CD15, Lewis-X, LeX, SSEA-1
Gene Symbol
FUT4
Additional CD15/Lewis X Products
Product Documents for CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free
Product Specific Notices for CD15/Lewis X Antibody (BRA-4F1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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