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CD173 Antibody (A51-B/A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14046

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgA Clone # A51-B/A6

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Human breast cancer MCF-7 cells

Epitope

Fuca1-2 Galb1-4 GlcNAc

Reactivity Notes

Others not known.

Localization

Cell Surface

Specificity

This antibody reacts with H type 2 blood group epitope Fuca1-2 Galb1-4 GlcNAc. It does not cross-react with human blood group H type 1, 3 or 4, nor with the closely related type 2 antigen Ley and Lex. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular trans- formation. Blood group related antigens are usually mucin-type and are detected on erythrocytes, certain epithelial cells and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A (1 and 2), B, H (1 and 2), M, N, Lewis A, Lewis B, Lewis X, Lewis Y and precursor type 1 chain antigens. The expressions of the H1 and H2 in different cell types are con- trolled by different genes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgA

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13931).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Applications for CD173 Antibody (A51-B/A6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistology (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

PEG purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD173

Alternate Names

H2, Type 2 H blood group antigen

Additional CD173 Products

Product Documents for CD173 Antibody (A51-B/A6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD173 Antibody (A51-B/A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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