CD177 Antibody (4C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00057126-M01

Novus Biologicals, part of Bio-Techne
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H00057126-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD177 Antibodies »

Product Specifications

Immunogen

CD177 (AAH29167, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ALLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLW

Specificity

CD177 (4C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD177 Antibody (4C4)

Western Blot: CD177 Antibody (4C4) [H00057126-M01]

Western Blot: CD177 Antibody (4C4) [H00057126-M01]

Western Blot: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody (M01), clone 4C4.Lane 1: CD177 transfected lysate(46.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01]

Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01]

Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01] - Analysis of monoclonal antibody to CD177 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01]

Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01]

Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD177 MaxPab rabbit polyclonal antibody.
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Applications for CD177 Antibody (4C4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD177

NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM]

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human)

Gene Symbol

CD177

OMIM

162860 (Human)

UniProt

AAH29167

Additional CD177 Products

Product Documents for CD177 Antibody (4C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD177 Antibody (4C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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