CD1a Antibody (O10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34697
Clone O10 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]](https://resources.bio-techne.com/images/products/CD1a-Antibody-O10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34697-img0003.jpg "Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rhesus Macaque
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # O10
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for CD1a Antibody (O10) - Azide and BSA Free
Immunogen
Human thymus cells
Localization
Cell surface
Marker
Mature Langerhans Cells Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34313).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD1a Antibody (O10) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10).![Flow Cytometry: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]](https://resources.bio-techne.com/images/products/CD1a-Antibody-O10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34697-img0002.jpg "Flow Cytometry: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]")
Flow Cytometry: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]
Flow Cytometry: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697] - Flow Cytometric Analysis of MOLT-4 cells using CD1a Antibody (O10) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).![Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]](https://resources.bio-techne.com/images/products/CD1a-Antibody-O10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34697-img0001.jpg "Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]")
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697]
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - Azide and BSA Free [NBP2-34697] - Human skin. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.Applications for CD1a Antibody (O10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-34697 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD1a
Long Name
T Cell Surface Glycoprotein CD1a
Alternate Names
CD1a, FCB6, HTA1, R4, T6
Gene Symbol
CD1A
Additional CD1a Products
Product Documents for CD1a Antibody (O10) - Azide and BSA Free
Product Specific Notices for CD1a Antibody (O10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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