CD20 Antibody (AISB12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43435

Novus Biologicals, part of Bio-Techne
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NBP1-43435-0.025mg
NBP1-43435-0.1mg
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Conjugate
Catalog #
Alexa Fluor 350
NBP1-43435AF350
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Alexa Fluor 405
NBP1-43435AF405
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Alexa Fluor 488
NBP1-43435AF488
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Alexa Fluor 532
NBP1-43435AF532
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Alexa Fluor 594
NBP1-43435AF594
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Alexa Fluor 647
NBP1-43435AF647
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Alexa Fluor 700
NBP1-43435AF700
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Alexa Fluor 750
NBP1-43435AF750
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Allophycocyanin
NBP1-43435APC
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Allophycocyanin/Cy7
NBP1-43435APCCY7
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Biotin
NBP1-43435B
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CoraFluor 1
NBP1-43435CL1
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DyLight 350
NBP1-43435UV
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DyLight 405
NBP1-43435V
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DyLight 488
NBP1-43435G
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DyLight 550
NBP1-43435R
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DyLight 594
NBP1-43435DL594
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DyLight 650
NBP1-43435C
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DyLight 680
NBP1-43435FR
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DyLight 755
NBP1-43435IR
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FITC
NBP1-43435F
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HRP
NBP1-43435H
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Janelia Fluor 525
NBP1-43435JF525
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Janelia Fluor 549
NBP1-43435JF549
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Janelia Fluor 585
NBP1-43435JF585
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Janelia Fluor 635
NBP1-43435JF635
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Janelia Fluor 646
NBP1-43435JF646
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Janelia Fluor 669
NBP1-43435JF669
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mFluor Violet 450 SE
NBP1-43435MFV450
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mFluor Violet 500 SE
NBP1-43435MFV500
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mFluor Violet 610 SE
NBP1-43435MFV610
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PE
NBP1-43435PE
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PE/Atto594
NBP1-43435PEATT594
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PE/Cy5.5
NBP1-43435PECY55
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PE/Cy7
NBP1-43435PECY7
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PerCP
NBP1-43435PCP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # AISB12

Format

BSA Free

Concentration

0.5 mg/ml

View all CD20 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD20.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD20 Antibody (AISB12) - BSA Free

Western Blot: CD20 Antibody (AISB12)BSA Free [NBP1-43435]

Western Blot: CD20 Antibody (AISB12)BSA Free [NBP1-43435]

Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.
Flow Cytometry: CD20 Antibody (AISB12) - BSA Free [NBP1-43435]

Flow Cytometry: CD20 Antibody (AISB12) - BSA Free [NBP1-43435]

Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.

Applications for CD20 Antibody (AISB12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000
Application Notes
This AISB12 antibody has been tested by immunoblot on A20 cell lysates and flow cytometry on mouse splenocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD20

CD20 is a non-glycosylated phosphoprotein that is expressed on the surface of normal and malignant B cells and functions in mediating calcium transport and B cell differentiation (1,2). CD20 is encoded by the membrane-spanning 4-domain family A member 1 (MS4A1) gene and, in humans, is located on chromosome 11q12 (1). The CD20 protein is 297 amino acids (aa) in length with a theoretical molecular weight (MW) of 33 kDa (1,2). Structurally, the CD20 protein has four membrane-spanning domains, two extracellular loop domains, and intracellular N- and C-terminal domains (1,2). CD20 is expressed at specific stages of B cell maturation including pre-B cells, mature naive and activated B cells, and memory B cells, but is absent from plasmablasts and plasma cells (1,3,4). Expression of CD20 is often increased on malignant B cells associated with various B cell disorders such as chronic lymphocytic leukemia (CLL), multiple sclerosis (MS), and rheumatoid arthritis (2-4). Anti-CD20 monoclonal antibody (mAb)-based therapies have become an appealing target for treating these immune-related disorders and cancers (1-5). Rituximab, a chimeric mAb, was the first FDA approved CD20 monoclonal antibody for the treatment of non-Hodgkin's lymphoma that is now commonly used to treat MS (2,3). Since its initial approval in 1997, several other chimeric and humanized anti-CD20 mAbs have been developed including Ofatumumab, Ublituximab, and Obinutuzumab (1-5). B cell depletion via CD20 mAbs can occur under different mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), complement-dependent cytotoxicity (CDC), and direct induction of apoptosis (1-4). Many ongoing studies are focused on combination therapies with CD20 mAbs as an addition to chemotherapy, B cell receptor (BCR) signaling inhibitors, or BH3 mimetics (1,2,4). Additionally, the effects of bispecific antibodies and CD20 chimeric antigen receptor (CAR) T cell therapies are under investigation for the treatment of B cell malignancies (4,5).

References

1. Pavlasova G, Mraz M. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020; 105(6):1494-1506. https://doi.org/10.3324/haematol.2019.243543

2. Payandeh Z, Bahrami AA, Hoseinpoor R, et al. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomed Pharmacother. 2019; 109:2415-2426. https://doi.org/10.1016/j.biopha.2018.11.121

3. Margoni M, Preziosa P, Filippi M, Rocca MA. Anti-CD20 therapies for multiple sclerosis: current status and future perspectives. J Neurol. 2022; 269(3):1316-1334. https://doi.org/10.1007/s00415-021-10744-x

4. Klein C, Jamois C, Nielsen T. Anti-CD20 treatment for B-cell malignancies: current status and future directions. Expert Opin Biol Ther. 2021; 21(2):161-181. https://doi.org/10.1080/14712598.2020.1822318

5. Sharman JP. Targeting CD20: teaching an old dog new tricks. Hematology Am Soc Hematol Educ Program. 2019; 2019(1):273-278. https://doi.org/10.1182/hematology.2019000031

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for CD20 Antibody (AISB12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD20 Antibody (AISB12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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