Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This monoclonal antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for CD20 Antibody (IGEL/773) - IHC-Prediluted

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335] - Human Lymphoma stained with CD20 Monoclonal Antibody (IGEL/773)

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335] - Formalin-fixed, paraffin-embedded human Tonsil.

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335] - Formalin-fixed, paraffin-embedded human Lymphoma

Applications for CD20 Antibody (IGEL/773) - IHC-Prediluted

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Multiplex Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD20

CD20 is a non-glycosylated phosphoprotein that is expressed on the surface of normal and malignant B cells and functions in mediating calcium transport and B cell differentiation (1,2). CD20 is encoded by the membrane-spanning 4-domain family A member 1 (MS4A1) gene and, in humans, is located on chromosome 11q12 (1). The CD20 protein is 297 amino acids (aa) in length with a theoretical molecular weight (MW) of 33 kDa (1,2). Structurally, the CD20 protein has four membrane-spanning domains, two extracellular loop domains, and intracellular N- and C-terminal domains (1,2). CD20 is expressed at specific stages of B cell maturation including pre-B cells, mature naive and activated B cells, and memory B cells, but is absent from plasmablasts and plasma cells (1,3,4). Expression of CD20 is often increased on malignant B cells associated with various B cell disorders such as chronic lymphocytic leukemia (CLL), multiple sclerosis (MS), and rheumatoid arthritis (2-4). Anti-CD20 monoclonal antibody (mAb)-based therapies have become an appealing target for treating these immune-related disorders and cancers (1-5). Rituximab, a chimeric mAb, was the first FDA approved CD20 monoclonal antibody for the treatment of non-Hodgkin's lymphoma that is now commonly used to treat MS (2,3). Since its initial approval in 1997, several other chimeric and humanized anti-CD20 mAbs have been developed including Ofatumumab, Ublituximab, and Obinutuzumab (1-5). B cell depletion via CD20 mAbs can occur under different mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), complement-dependent cytotoxicity (CDC), and direct induction of apoptosis (1-4). Many ongoing studies are focused on combination therapies with CD20 mAbs as an addition to chemotherapy, B cell receptor (BCR) signaling inhibitors, or BH3 mimetics (1,2,4). Additionally, the effects of bispecific antibodies and CD20 chimeric antigen receptor (CAR) T cell therapies are under investigation for the treatment of B cell malignancies (4,5).

References

1. Pavlasova G, Mraz M. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020; 105(6):1494-1506. https://doi.org/10.3324/haematol.2019.243543

2. Payandeh Z, Bahrami AA, Hoseinpoor R, et al. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomed Pharmacother. 2019; 109:2415-2426. https://doi.org/10.1016/j.biopha.2018.11.121

3. Margoni M, Preziosa P, Filippi M, Rocca MA. Anti-CD20 therapies for multiple sclerosis: current status and future perspectives. J Neurol. 2022; 269(3):1316-1334. https://doi.org/10.1007/s00415-021-10744-x

4. Klein C, Jamois C, Nielsen T. Anti-CD20 treatment for B-cell malignancies: current status and future directions. Expert Opin Biol Ther. 2021; 21(2):161-181. https://doi.org/10.1080/14712598.2020.1822318

5. Sharman JP. Targeting CD20: teaching an old dog new tricks. Hematology Am Soc Hematol Educ Program. 2019; 2019(1):273-278. https://doi.org/10.1182/hematology.2019000031

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for CD20 Antibody (IGEL/773) - IHC-Prediluted

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD20 Antibody (IGEL/773) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD20 Antibody (IGEL/773) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48335

Key Product Details

Species Reactivity

Validated:

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Antibody Source

Format

Concentration

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Product Summary for CD20 Antibody (IGEL/773) - IHC-Prediluted

Immunogen

Reactivity Notes

Localization

Specificity

Marker

Clonality

Host

Isotype

Description

Scientific Data Images for CD20 Antibody (IGEL/773) - IHC-Prediluted

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335]

Immunohistochemistry-Paraffin: CD20 Antibody (IGEL/773) - IHC-Prediluted [NBP2-48335]

Applications for CD20 Antibody (IGEL/773) - IHC-Prediluted

Immunohistochemistry

Immunohistochemistry-Paraffin

Multiplex Immunoassay

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD20

Long Name

Alternate Names

Gene Symbol

Additional CD20 Products

Product Documents for CD20 Antibody (IGEL/773) - IHC-Prediluted

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD20 Antibody (IGEL/773) - IHC-Prediluted