CD20 Antibody (MS4A1/6993R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24154

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-24154
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # MS4A1/6993R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD20 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 97-297) corresponding to C-terminal of human CD20 protein (exact sequence is proprietary)

Reactivity Notes

Expected to react with Monkey, Baboon, Gorilla, Dog and Chimpanzee.

Localization

Cell surface.

Specificity

This monoclonal antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD20 Antibody (MS4A1/6993R) - Azide and BSA Free

CD20 Antibody (MS4A1/6993R) - Azide and BSA Free Western Blot: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] -

Western Blot: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] -

Western Blot: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] - Western blot analysis of Raji cell lysate using CD20 Antibody (MS4A1/6993R)
CD20 Antibody (MS4A1/6993R) - Azide and BSA Free Immunohistochemistry-Paraffin: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] -

Immunohistochemistry-Paraffin: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] -

Immunohistochemistry-Paraffin: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] - Formalin-fixed, paraffin-embedded human kidney stained with CD20 Antibody (MS4A1/6993R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
CD20 Antibody (MS4A1/6993R) - Azide and BSA Free Immunohistochemistry-Paraffin: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] -

Immunohistochemistry-Paraffin: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] -

Immunohistochemistry-Paraffin: CD20 Antibody (MS4A1/6993R) - Azide and BSA Free [NBP3-24154] - Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Antibody (MS4A1/6993R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for CD20 Antibody (MS4A1/6993R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD20

CD20 is a non-glycosylated phosphoprotein that is expressed on the surface of normal and malignant B cells and functions in mediating calcium transport and B cell differentiation (1,2). CD20 is encoded by the membrane-spanning 4-domain family A member 1 (MS4A1) gene and, in humans, is located on chromosome 11q12 (1). The CD20 protein is 297 amino acids (aa) in length with a theoretical molecular weight (MW) of 33 kDa (1,2). Structurally, the CD20 protein has four membrane-spanning domains, two extracellular loop domains, and intracellular N- and C-terminal domains (1,2). CD20 is expressed at specific stages of B cell maturation including pre-B cells, mature naive and activated B cells, and memory B cells, but is absent from plasmablasts and plasma cells (1,3,4). Expression of CD20 is often increased on malignant B cells associated with various B cell disorders such as chronic lymphocytic leukemia (CLL), multiple sclerosis (MS), and rheumatoid arthritis (2-4). Anti-CD20 monoclonal antibody (mAb)-based therapies have become an appealing target for treating these immune-related disorders and cancers (1-5). Rituximab, a chimeric mAb, was the first FDA approved CD20 monoclonal antibody for the treatment of non-Hodgkin's lymphoma that is now commonly used to treat MS (2,3). Since its initial approval in 1997, several other chimeric and humanized anti-CD20 mAbs have been developed including Ofatumumab, Ublituximab, and Obinutuzumab (1-5). B cell depletion via CD20 mAbs can occur under different mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), complement-dependent cytotoxicity (CDC), and direct induction of apoptosis (1-4). Many ongoing studies are focused on combination therapies with CD20 mAbs as an addition to chemotherapy, B cell receptor (BCR) signaling inhibitors, or BH3 mimetics (1,2,4). Additionally, the effects of bispecific antibodies and CD20 chimeric antigen receptor (CAR) T cell therapies are under investigation for the treatment of B cell malignancies (4,5).

References

1. Pavlasova G, Mraz M. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020; 105(6):1494-1506. https://doi.org/10.3324/haematol.2019.243543

2. Payandeh Z, Bahrami AA, Hoseinpoor R, et al. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomed Pharmacother. 2019; 109:2415-2426. https://doi.org/10.1016/j.biopha.2018.11.121

3. Margoni M, Preziosa P, Filippi M, Rocca MA. Anti-CD20 therapies for multiple sclerosis: current status and future perspectives. J Neurol. 2022; 269(3):1316-1334. https://doi.org/10.1007/s00415-021-10744-x

4. Klein C, Jamois C, Nielsen T. Anti-CD20 treatment for B-cell malignancies: current status and future directions. Expert Opin Biol Ther. 2021; 21(2):161-181. https://doi.org/10.1080/14712598.2020.1822318

5. Sharman JP. Targeting CD20: teaching an old dog new tricks. Hematology Am Soc Hematol Educ Program. 2019; 2019(1):273-278. https://doi.org/10.1182/hematology.2019000031

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for CD20 Antibody (MS4A1/6993R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD20 Antibody (MS4A1/6993R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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