CD200/OX2 Antibody (PSH01-48)

Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132]

Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution.

Lane 1: SK-MEL-28 cell lysate (20 ug/Lane)
Lane 2: NCI-H226 cell lysate (20 ug/Lane)
Lane 3: Human brain tissue lysate (30 ug/Lane)
Lane 4: Human placenta tissue lysate (30 ug/Lane)
Lane 5: Human lung tissue lysate (30 ug/Lane)

Predicted band size: 31 kDa
Observed band size: 40-50kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132]

Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD200/OX2 with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32132, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132]

Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.