Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CR2/1952

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD21 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 44-196) of human CR2 (CD21) protein (exact sequence is proprietary) (Uniprot: P20023)

Localization

Cell surface

Marker

Mature B-Cell & Follicular Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-60732).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD21 Antibody (CR2/1952) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733]

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733]

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733] - Formalin-fixed, paraffin-embedded human Follicular Dendritic Cell Sarcoma stained with CD21 Antibody (CR2/1952).
Immunohistochemistry-Paraffin: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733]

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733]

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733] - Formalin-fixed, paraffin-embedded Human Tonsil stained with CD21 Mouse Monoclonal Antibody (CR2/1952).
Protein Array: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733]

Protein Array: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733]

Protein Array: CD21 Antibody (CR2/1952) - Azide and BSA Free [NBP2-60733] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD21 Antibody (CR2/1952) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for CD21 Antibody (CR2/1952) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD21

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Alternate Names

C3DR, CD21, CR2

Gene Symbol

CR2

UniProt

P20023 (Human)

Additional CD21 Products

Product Documents for CD21 Antibody (CR2/1952) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD21 Antibody (CR2/1952) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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