CD21 Antibody (CR2/3124R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08297

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-08297
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # CR2/3124R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD21 Antibodies »

Product Summary for CD21 Antibody (CR2/3124R) - Azide and BSA Free

Immunogen

Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary) (Uniprot: P20023)

Localization

Cell Surface

Marker

Mature B-Cell & Follicular Dendritic Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07754).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD21 Antibody (CR2/3124R) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297]

Immunocytochemistry/ Immunofluorescence: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297]

Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.
Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297]

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297]

Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297] - Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).
Flow Cytometry: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297]

Flow Cytometry: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297]

Flow Cytometry: CD21 Antibody (CR2/3124R) - Azide and BSA Free [NBP3-08297] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Applications for CD21 Antibody (CR2/3124R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD21

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Alternate Names

C3DR, CD21, CR2

Gene Symbol

CR2

Additional CD21 Products

Product Documents for CD21 Antibody (CR2/3124R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD21 Antibody (CR2/3124R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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