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CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50070

Novus Biologicals, part of Bio-Techne
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NBP1-50070
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HLy9.25

Format

BSA Free

Concentration

1 mg/ml

Product Summary for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

Immunogen

CD299-transfected 300.19 pre-B cell line

Specificity

The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes Lymphocyte Antigen 9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free [NBP1-50070]

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free [NBP1-50070]

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) PE.
Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free [NBP1-50070]

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free [NBP1-50070]

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) APC.
CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free [NBP1-50070]

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free [NBP1-50070]

Surface staining pattern of human peripheral blood stained using anti-human CD229 (HLy9.25) purified antibody (concentration in sample 16 ug/ml) GAM APC.

Applications for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

Application
Recommended Usage

Flow Cytometry

6 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Functional Application: Regulation of activation. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD229/SLAMF3

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Long Name

SLAM Family Member 3

Alternate Names

CD229, Ly9, SLAMF3

Gene Symbol

LY9

Additional CD229/SLAMF3 Products

Product Documents for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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