CD23/Fc epsilon RII Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90682

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD23/Fc epsilon RII Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD23/Fc epsilon RII (Uniprot#: P20693-1; Glu51-Pro331).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD23/Fc epsilon RII Antibody (007)

Immunocytochemistry/ Immunofluorescence: CD23/Fc epsilon RII Antibody (007) [NBP2-90682]

Immunocytochemistry/ Immunofluorescence: CD23/Fc epsilon RII Antibody (007) [NBP2-90682]

Immunocytochemistry/Immunofluorescence: CD23/Fc epsilon RII Antibody (007) [NBP2-90682] - Immunofluorescence staining of mouse CD23/Fc epsilon RII in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD23/Fc epsilon RII monoclonal antibody (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).
Flow Cytometry: CD23/Fc epsilon RII Antibody (007) [NBP2-90682]

Flow Cytometry: CD23/Fc epsilon RII Antibody (007) [NBP2-90682]

Flow Cytometry: CD23/Fc epsilon RII Antibody (007) [NBP2-90682] - Flow cytometric analysis of Mouse CD23/Fc epsilon RII expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD23/Fc epsilon RII, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for CD23/Fc epsilon RII Antibody (007)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD23/Fc epsilon RII

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. CD23 is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a

Long Name

Fc epsilon Receptor II

Alternate Names

CD23, CLEC4J, Fc epsilon RII, FCER2, Fcer2a, FceRII, IGEBF, Ly-42

Gene Symbol

FCER2

Additional CD23/Fc epsilon RII Products

Product Documents for CD23/Fc epsilon RII Antibody (007)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD23/Fc epsilon RII Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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