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CD24 Antibody (4F4E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37352

Novus Biologicals, part of Bio-Techne
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NBP2-37352-0.025ml
NBP2-37352

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F4E10

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human CD24 (AA: 15-80) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD24 Antibody (4F4E10) - BSA Free

Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis using CD24 mAb against HEK293 (1) and CD24 (AA: 15-80)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD24 Antibody (4F4E10) - BSA Free [NBP2-37352]

Flow Cytometry: CD24 Antibody (4F4E10) - BSA Free [NBP2-37352]

Flow Cytometry: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis of Hela cells using CD24 mouse mAb (green) and negative control (red).
Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis using CD24 mAb against human CD24 recombinant protein. (Expected MW is 32.1 kDa)

Applications for CD24 Antibody (4F4E10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD24

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Alternate Names

CD24, CD24A

Gene Symbol

CD24

Additional CD24 Products

Product Documents for CD24 Antibody (4F4E10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD24 Antibody (4F4E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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