CD24 Antibody (ML5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80636

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ML5

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD24 Antibodies »

Product Specifications

Immunogen

The immunogen was the full length version of the protein: CD24 (ML5)

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD24 Antibody (ML5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]

Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]

Immunocytochemistry/Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - MCF-7 breast cancer cells. Image from the standard format of this antibody.
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]

Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]

Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - Analysis using the FITC conjugate of NB100-77903. Staining of Human peripheral blood lymphocytes stained ML5 FITC.
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]

Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]

Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - Whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC Image from the standard format of this antibody.

Applications for CD24 Antibody (ML5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

0.125 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

5-25 ug/ml
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD24

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Alternate Names

CD24, CD24A

Gene Symbol

CD24

Additional CD24 Products

Product Documents for CD24 Antibody (ML5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD24 Antibody (ML5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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