CD24 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46390

Novus Biologicals, part of Bio-Techne
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NBP1-46390
NBP1-46390SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CD24 Antibodies »

Product Specifications

Immunogen

A synthetic peptide from aa region 30-70 of human mature Signal transducer CD24 conjugated to blue carrier protein was used as the antigen.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23301003).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD24 Antibody

Immunocytochemistry/ Immunofluorescence: CD24 Antibody [NBP1-46390]

Immunocytochemistry/ Immunofluorescence: CD24 Antibody [NBP1-46390]

Immunocytochemistry/Immunofluorescence: CD24 Antibody [NBP1-46390] - Mouse WAT-CM induces the expression of CD24 in mesenchymal MDA-MB-231 cells but does not affect the expression of vimentin. Representative images of MDA-MB-231 cells cultured with or without lean or obese subcutaneous or visceral WAT-CM and co-stained for vimentin, CD24, and DAPI (a); scale bar = 100 uM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899433/) licensed under a CC-BY license.

Applications for CD24 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:500-1:1000
Application Notes
The optimal dilution should be determined by the end user. Not tested in other applications. ICC/IF was reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 31040372).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD24

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Alternate Names

CD24, CD24A

Entrez Gene IDs

100133941 (Human)

Gene Symbol

CD24

UniProt

P25063

Additional CD24 Products

Product Documents for CD24 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD24 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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