CD25/IL-2R alpha Antibody (143-13) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47725F
Clone 143-13 was used by HLDA to establish CD designation.
![CD25/IL-2R alpha Antibody (143-13) [FITC]](https://resources.bio-techne.com/images/products/nbp2-47725f_mouse-monoclonal-cd25-il-2r-alpha-antibody-143-13-fitc-31720238131590.jpg "CD25/IL-2R alpha Antibody (143-13) [FITC] [NBP2-47725F] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 143-13
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Stimulated human leukocytes
Localization
Cell Surface
Specificity
Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This monoclonal antibody recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
Marker
Activated Lymphocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Scientific Data Images for CD25/IL-2R alpha Antibody (143-13) [FITC]
CD25/IL-2R alpha Antibody (143-13) [FITC] [NBP2-47725F] -
CD25/IL-2R alpha Antibody (143-13) [FITC] [NBP2-47725F] - Vial of FITC conjugated antibody. FITC is optimally excited at 498 nm by the Blue laser (488 nm) and has an emission maximum of 519 nm.Applications for CD25/IL-2R alpha Antibody (143-13) [FITC]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Gene Symbol
IL2RA
Additional CD25/IL-2R alpha Products
Product Documents for CD25/IL-2R alpha Antibody (143-13) [FITC]
Product Specific Notices for CD25/IL-2R alpha Antibody (143-13) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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