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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Alexa Fluor 532

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 280406

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
Glu22-Lys236
Accession # P01590

Specificity

Detects mouse CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) gammac, recombinant human CD25/IL‑2 R alpha, rmIL-2 R beta, or rmIL-15 R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532]

CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532]

CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532] [FAB2438X] -

CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532] [FAB2438X] - Vial of Alexa Fluor 532 conjugated antibody. Alexa Fluor 532 is optimally excited at 532 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 554 nm.

Applications for CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD25/IL-2R alpha Antibody (280406) [Alexa Fluor® 532]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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