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CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-710AF488

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # PC61.5.3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488]

Immunogen

B6.1 CTL cell line.

Localization

Cell Membrane, Cytoplasm

Specificity

This reacts with the low affinity alpha chain of the IL2 receptor present on activated T and B cells in mice. Clone PC61.5.3 is reported to inhibit IL2 binding and IL2 dependent proliferation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488]

Product Image: CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488] [NB600-710AF488] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.

Applications for CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD25/IL-2R alpha Antibody (PC61.5.3) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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