CD27 Ligand/TNFSF7/CD70 Antibody (BU69)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44545
Clone BU69 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BU69
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human WM-1 (Waldenstrom s macroglobulinemia) cell line
Localization
Cell Surface
Specificity
It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This monoclonal antibody blocks the interaction between CD27 and CD70, and has been shown to inhibit T cell proliferation induced by dendritic cells.
Marker
Activated T- & B-Lymphocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.(NBP3-11586)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for CD27 Ligand/TNFSF7/CD70 Antibody (BU69)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Frozen
1-2 ug/ml
Immunoprecipitation
1-2 ug/500 ug protein lysate
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Inhibits T cell proliferation induced by dendritic cells: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Inhibits T cell proliferation induced by dendritic cells: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD27 Ligand/TNFSF7
Additional CD27 Ligand/TNFSF7 Products
Product Documents for CD27 Ligand/TNFSF7/CD70 Antibody (BU69)
Product Specific Notices for CD27 Ligand/TNFSF7/CD70 Antibody (BU69)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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