CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54582
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]](https://resources.bio-techne.com/images/products/CD27-TNFRSF7-Antibody-LPFS2-1611-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54582-img0004.jpg "Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]")
![Immunocytochemistry/ Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]](https://resources.bio-techne.com/images/products/CD27-TNFRSF7-Antibody-LPFS2-1611-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54582-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LPFS2/1611
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free
Immunogen
Recombinant full-length human CD27/TNFRSF7 protein (Uniprot: P26842)
Localization
Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53196).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]
Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]](https://resources.bio-techne.com/images/products/CD27-TNFRSF7-Antibody-LPFS2-1611-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54582-img0006.jpg "Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]")
Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]
Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27/TNFRSF7 Antibody (LPFS2/1611).![Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]](https://resources.bio-techne.com/images/products/CD27-TNFRSF7-Antibody-LPFS2-1611-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54582-img0007.jpg "Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]")
Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]
Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582] - Flow Cytometric Analysis of Ramos cells using CD27/TNFRSF7 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]](https://resources.bio-techne.com/images/products/CD27-TNFRSF7-Antibody-LPFS2-1611-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54582-img0005.jpg "Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]")
![Protein Array: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]](https://resources.bio-techne.com/images/products/CD27-TNFRSF7-Antibody-LPFS2-1611-Azide-and-BSA-Free-Protein-Array-NBP2-54582-img0002.jpg "Protein Array: CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free [NBP2-54582]")
Applications for CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD27/TNFRSF7
Membrane-bound CD27 is expressed as a disulfide-linked homodimer (3). CD27 binds to the ligand CD70, a transmembrane glycoprotein that is transiently expressed on activated immune cells such as antigen presenting cells (APCs), dendritic cells (DCs), NK cells, B cells, and T cells (1,2,6,7). The receptor-ligand binding interaction leads to NFkappaB and c-Jun pathway activation which promotes immune stimulation and activation and survival of CD4+ T cells, CD8+ T cells, memory T cells, and NK cells (2,6,7). Both CD27 and CD70 are often abnormally expressed or dysregulated on malignant and cancer cells leading to immune evasion and tumor progression (7). CD27 has become a target of interest of immunotherapies for viral infections, autoimmune disease, and cancer (2). Varlilumab, an agonistic CD27 monoclonal antibody (mAB), has entered clinical trials for the treatment of hematological and solid tumor cancers (1,6). Additional clinical trials are in process that combine varlilumab with other immune checkpoint inhibitors like the programmed cell death protein-1 (PD-1) blocking mAb nivolumab (1,2). Initial results are promising, suggesting that targeting CD27, especially in combination with other therapeutics, may be a promising and effective immunotherapy for a variety of pathologies (1,2,6).
References
1. Starzer AM, Berghoff AS. New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). ESMO Open. 2020;4(Suppl 3):e000629. https://doi.org/10.1136/esmoopen-2019-000629
2. Grant EJ, Nussing S, Sant S, Clemens EB, Kedzierska K. The role of CD27 in anti-viral T-cell immunity. Curr Opin Virol. 2017;22:77-88. https://doi.org/10.1016/j.coviro.2016.12.001
3. Buchan SL, Rogel A, Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy. Blood. 2018;131(1):39-48. https://10.1182/blood-2017-07-741025
4. Uniprot (P26842)
5. Uniprot (P41272)
6. van de Ven K, Borst J. Targeting the T-cell co-stimulatory CD27/CD70 pathway in cancer immunotherapy: rationale and potential. Immunotherapy. 2015;7(6):655-667. https://doi.org/10.2217/imt.15.32
7. Flieswasser T, Van den Eynde A, Van Audenaerde J, et al. The CD70-CD27 axis in oncology: the new kids on the block. J Exp Clin Cancer Res. 2022;41(1):12. https://doi.org/10.1186/s13046-021-02215-y
Alternate Names
CD27, TNFRSF7
Gene Symbol
CD27
Additional CD27/TNFRSF7 Products
Product Documents for CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free
Product Specific Notices for CD27/TNFRSF7 Antibody (LPFS2/1611) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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