Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the PE/Cy7 conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (open histogram) or Anti-Human CD27 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the Allophycocyanin conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (open histogram) or Anti-Human CD27 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426] - Analysis using the Biotin conjugate of NBP1-43426. Staining of normal human peripheral blood cells with Anti-Human CD27 PE (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

View 3 more images

Applications for CD27/TNFRSF7 Antibody (O323)

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

The O323 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD27/TNFRSF7

CD27, also referred to as tumor necrosis factor receptor superfamily, member 7 (TNFRSF7), is a type I transmembrane glycoprotein that functions as a co-stimulatory T cell receptor and is expressed on the surface of T cells, natural killer (NK) cells, and B cells (1,2). The human CD27 protein is 260 amino acids (aa) in length and consists of a 19 aa signal sequence, 172 aa extracellular domain (ECD) containing three characteristic cysteine-rich domains (CRDs), a 21 aa helical transmembrane region, and a 48 aa cytoplasmic tail domain (3,4). The CD27 protein has a theoretical molecular weight (MW) of 29 kDa, but is typically is closer to 50-55 kDa due to N-linked and O-linked glycosylation (3). Mouse CD27 cDNA encodes a 250 aa protein with a theoretical molecular weight of 28 kDa (5). Human CD27 shares ~64% aa sequence identity with mouse CD27 protein.

Membrane-bound CD27 is expressed as a disulfide-linked homodimer (3). CD27 binds to the ligand CD70, a transmembrane glycoprotein that is transiently expressed on activated immune cells such as antigen presenting cells (APCs), dendritic cells (DCs), NK cells, B cells, and T cells (1,2,6,7). The receptor-ligand binding interaction leads to NFkappaB and c-Jun pathway activation which promotes immune stimulation and activation and survival of CD4+ T cells, CD8+ T cells, memory T cells, and NK cells (2,6,7). Both CD27 and CD70 are often abnormally expressed or dysregulated on malignant and cancer cells leading to immune evasion and tumor progression (7). CD27 has become a target of interest of immunotherapies for viral infections, autoimmune disease, and cancer (2). Varlilumab, an agonistic CD27 monoclonal antibody (mAB), has entered clinical trials for the treatment of hematological and solid tumor cancers (1,6). Additional clinical trials are in process that combine varlilumab with other immune checkpoint inhibitors like the programmed cell death protein-1 (PD-1) blocking mAb nivolumab (1,2). Initial results are promising, suggesting that targeting CD27, especially in combination with other therapeutics, may be a promising and effective immunotherapy for a variety of pathologies (1,2,6).

References

1. Starzer AM, Berghoff AS. New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). ESMO Open. 2020;4(Suppl 3):e000629. https://doi.org/10.1136/esmoopen-2019-000629

2. Grant EJ, Nussing S, Sant S, Clemens EB, Kedzierska K. The role of CD27 in anti-viral T-cell immunity. Curr Opin Virol. 2017;22:77-88. https://doi.org/10.1016/j.coviro.2016.12.001

3. Buchan SL, Rogel A, Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy. Blood. 2018;131(1):39-48. https://10.1182/blood-2017-07-741025

4. Uniprot (P26842)

5. Uniprot (P41272)

6. van de Ven K, Borst J. Targeting the T-cell co-stimulatory CD27/CD70 pathway in cancer immunotherapy: rationale and potential. Immunotherapy. 2015;7(6):655-667. https://doi.org/10.2217/imt.15.32

7. Flieswasser T, Van den Eynde A, Van Audenaerde J, et al. The CD70-CD27 axis in oncology: the new kids on the block. J Exp Clin Cancer Res. 2022;41(1):12. https://doi.org/10.1186/s13046-021-02215-y

Alternate Names

CD27, TNFRSF7

Gene Symbol

CD27

Additional CD27/TNFRSF7 Products

Product Documents for CD27/TNFRSF7 Antibody (O323)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD27/TNFRSF7 Antibody (O323)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CD27/TNFRSF7 Antibody (O323)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43426

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all CD27/TNFRSF7 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for CD27/TNFRSF7 Antibody (O323)

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Flow Cytometry: CD27/TNFRSF7 Antibody (O323) [NBP1-43426]

Applications for CD27/TNFRSF7 Antibody (O323)

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CD27/TNFRSF7

Alternate Names

Gene Symbol

Additional CD27/TNFRSF7 Products

Product Documents for CD27/TNFRSF7 Antibody (O323)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD27/TNFRSF7 Antibody (O323)