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CD2BP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1526

Novus Biologicals, part of Bio-Techne
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NB100-1526

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence PKRKVTFQGVGDE-C corresponding to N-Terminus according to NP_006101.1.

Epitope

N Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CD2BP2 Antibody

Western Blot: CD2BP2 Antibody [NB100-1526]

Western Blot: CD2BP2 Antibody [NB100-1526]

Western Blot: CD2BP2 Antibody [NB100-1526] - (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for CD2BP2 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 10 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human lymph node, thymus and tonsil lysates (calculated MW of 37.6 kDa band according to NP_006101.1). The observed molecular weights correspond to earlier findings is reported in scientific literature with different antibodies (Nishizawa et al, Proc Natl Acad Sci U S A. 1998 Dec 8;95(25): 14897-902). IHC-P: Human spleen shows nucleolar staining in select splenocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD2BP2

CD2BP2 is an intracellular protein which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17 amino acid motif also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over expression of the isolated CD2BP2 domain binding to CD2 enhances the production of interleukin 2 on crosslinking of CD2 but not the T cell receptor.

Alternate Names

CD2 (cytoplasmic tail) binding protein 2, CD2 antigen (cytoplasmic tail) binding protein 2, CD2 antigen (cytoplasmic tail)-binding protein 2, CD2 antigen cytoplasmic tail-binding protein 2, CD2 binding protein 2, CD2 cytoplasmic domain binding protein 2, CD2 cytoplasmic domain-binding protein 2, CD2 tail-binding protein 2, FWP010, KIAA1178, LIN1, Snu40, U5 snRNP 52K protein, U5-52K

Entrez Gene IDs

10421 (Human); 70233 (Mouse)

Gene Symbol

CD2BP2

Additional CD2BP2 Products

Product Documents for CD2BP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD2BP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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