CD3 delta Antibody (6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89729

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 delta/CD3d Antibodies »

Product Summary for CD3 delta Antibody (6)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3 delta (Accession#: NP_000723.1; Met 1-Ala 105).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD3 delta Antibody (6)

Western Blot: CD3 delta Antibody (6) [NBP2-89729]

Western Blot: CD3 delta Antibody (6) [NBP2-89729]

Western Blot: CD3 delta Antibody (6) [NBP2-89729] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:19 kDa Observed band size:25 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: CD3 delta Antibody (6) [NBP2-89729]

Immunoprecipitation: CD3 delta Antibody (6) [NBP2-89729]

Immunoprecipitation: CD3 delta Antibody (6) [NBP2-89729] - Lane A:0.5 mg HuT78 Whole Cell Lysate 0.5 uL anti-CD3D rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD3D rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size: 20 kDa

Applications for CD3 delta Antibody (6)

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 delta/CD3d

CD3 delta is encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq]

Long Name

CD3 delta

Alternate Names

CD3D

Gene Symbol

CD3D

Additional CD3 delta/CD3d Products

Product Documents for CD3 delta Antibody (6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 delta Antibody (6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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