CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52641
Recombinant Monoclonal Antibody.
![Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-145-2C11-Flow-Cytometry-NBP2-52641-img0004.jpg "Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]")
![Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-145-2C11-Flow-Cytometry-NBP2-52641-img0005.jpg "Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]")
![Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-145-2C11-Flow-Cytometry-NBP2-52641-img0002.jpg "Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Functional, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 145-2C11
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone.
Reactivity Notes
Rat
Specificity
Recognises a 25-kDa protein component (CD3e, originally called T3-e) of the antigen-specific T-cell receptor.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free
Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]
Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric [NBP2-52641] - CD3 epsilon Antibody (145-2C11) [NBP2-52641] - Analysis using anti-CD3 epsilon and TRP1 antibodies. Mouse splenocytes.![Western Blot: CD3 epsilon Antibody (145-2C11)ChimericAzide and BSA Free [NBP2-52641]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-145-2C11-Western-Blot-NBP2-52641-img0001.jpg "Western Blot: CD3 epsilon Antibody (145-2C11)ChimericAzide and BSA Free [NBP2-52641]")
Western Blot: CD3 epsilon Antibody (145-2C11)ChimericAzide and BSA Free [NBP2-52641]
Western Blot: CD3 epsilon Antibody (145-2C11) [NBP2-52641] - Rat lymph node (lane 1), rat rectum (lane 2), rat spleen (lane 3), mouse lung (lane 4)and mouse pancreas (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with Anti-CD3 epsilon-1.4 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (26 kDa) is seen for both rat and mouse samples.![Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-145-2C11-Flow-Cytometry-NBP2-52641-img0006.jpg "Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]")
Flow Cytometry: CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free [NBP2-52641]
Flow Cytometry: CD3 epsilon Antibody (145-2C11) [NBP2-52641] - Analysis using anti-CD3 epsilon and TRP1 antibodies. KPC3 cells transfected with the Trp1 gene.Applications for CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD3 epsilon
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free
Product Specific Notices for CD3 epsilon Antibody (145-2C11) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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