CD3 epsilon Antibody (APA1/1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30426
![Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-APA1-1-Flow-Cytometry-NBP1-30426-img0002.jpg "Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # APA1/1
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for CD3 epsilon Antibody (APA1/1) - BSA Free
Immunogen
Purified human CD3 proteins isolated from thymus
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD3 epsilon Antibody (APA1/1) - BSA Free
Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]
Flow Cytometry: CD3 epsilon Antibody (APA1/1) [NBP1-30426] - Intracellular staining of JURKAT cells with anti-CD3 epsilon activation epitope (APA1/1) PE.![Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-APA1-1-Flow-Cytometry-NBP1-30426-img0001.jpg "Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]")
Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]
Flow Cytometry: CD3 epsilon Antibody (APA1/1) [NBP1-30426] - Intracellular staining of human peripheral blood with anti-CD3 epsilon activation epitope (APA1/1) purified, GAM-FITC.![CD3 epsilon Antibody (APA1/1) - BSA Free Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]](https://resources.bio-techne.com/images/products/nbp1-30426_mouse-monoclonal-cd3-epsilon-antibody-apa1-1-13120241616151.jpg "Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]")
Flow Cytometry: CD3 epsilon Antibody (APA1/1) - BSA Free [NBP1-30426]
Separation of Jurkat cells stained anti-human CD3 activation epitope (APA1/1) purified antibody (concentration in sample 5 ug/ml, GAM FITC, red-filled) from Jurkat cells unstained by primary antibody (GAM FITC, black-dashed) in Analysis (intracellular staining).Applications for CD3 epsilon Antibody (APA1/1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunoprecipitation
1:50
Western Blot
1:1000
Application Notes
Flow Cytometry: Sample preparation - At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 ug/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. Immunocytochemistry: Fixed and permeabilized cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD3 epsilon
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (APA1/1) - BSA Free
Product Specific Notices for CD3 epsilon Antibody (APA1/1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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