CD3 epsilon Antibody (BL-298-5D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76424

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-298-5D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 157 and 207

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 epsilon Antibody (BL-298-5D12)

Immunocytochemistry/ Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424]

Immunocytochemistry/ Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424]

Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
Western Blot: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424]

Western Blot: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424]

Western Blot: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of Jurkat, MOLT-4, K-562, SK-MEL-28 (SK), and HeLa lysate Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin.
Immunohistochemistry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424]

Immunohistochemistry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424]

Immunohistochemistry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for CD3 epsilon Antibody (BL-298-5D12)

Application
Recommended Usage

Flow Cytometry

1 ul per 1 X 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD3 epsilon

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The epsilon polypeptide plays an essential role in T-cell development.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (BL-298-5D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 epsilon Antibody (BL-298-5D12)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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