CD3 epsilon Antibody (CL1466)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34483

Novus Biologicals, part of Bio-Techne
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NBP2-34483
NBP2-34483-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1466

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMD

Epitope

IGSDEDHLSLKEFSE

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD3 epsilon Antibody (CL1466)

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (CL1466) [NBP2-34483]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (CL1466) [NBP2-34483]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (CL1466) [NBP2-34483] - Staining in human tonsil and skeletal muscle tissues. Corresponding CD3E RNA-seq data are presented for the same tissues.
Western Blot: CD3 epsilon Antibody (CL1466) [NBP2-34483]

Western Blot: CD3 epsilon Antibody (CL1466) [NBP2-34483]

Western Blot: CD3 epsilon Antibody (CL1466) [NBP2-34483] - Lane 1: Marker [kDa]. Lane 2: Human tonsil tissue lysate
Immunohistochemistry-Paraffin: CD3 epsilon Antibody (CL1466) [NBP2-34483]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (CL1466) [NBP2-34483]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (CL1466) [NBP2-34483] - Staining of human tonsil shows strong membranous positivity, mainly in in non - germinal center cells.
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Applications for CD3 epsilon Antibody (CL1466)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

UniProt

P07766

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (CL1466)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (CL1466)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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