CD3 epsilon Antibody (PC3/188A)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53386
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0006.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]")
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0007.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Chimpanzee
Cited:
Goat
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative), Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PC3/188A
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain (Uniprot: P07766 )
Reactivity Notes
Others not known.
Localization
Cell surface and Cytoplasmic
Specificity
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54405)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for CD3 epsilon Antibody (PC3/188A)
Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]
Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-PC3-188A-Immunohistochemistry-Paraffin-NBP2-53386-img0001.jpg "Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]")
Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]
Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0005.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]")
Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386]
Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Thymus lysate using Purified CD3 epsilon Antibody (PC3/188A).Applications for CD3 epsilon Antibody (PC3/188A)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD3 epsilon
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (PC3/188A)
Product Specific Notices for CD3 epsilon Antibody (PC3/188A)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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