CD3 epsilon Antibody (RIV9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11471
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # RIV9
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human peripheral lymphocytes (Uniprot: P07766)
Localization
Cell surface and cytoplasmic
Specificity
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This monoclonal antibody is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44880).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD3 epsilon Antibody (RIV9) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD3 epsilon Antibody (RIV9) - Azide and BSA Free [NBP3-11471]
Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (RIV9) - Azide and BSA Free [NBP3-11471] - Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3 epsilon antibody (RIV9) (green), mounted in EverBrite Mounting Medium with DAPI (nuclei, blue).Flow Cytometry: CD3 epsilon Antibody (RIV9) - Azide and BSA Free [NBP3-11471]
Flow Cytometry: CD3 epsilon Antibody (RIV9) - Azide and BSA Free [NBP3-11471] - Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 epsilon antibody (RIV9) (green)Applications for CD3 epsilon Antibody (RIV9) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD3 epsilon
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (RIV9) - Azide and BSA Free
Product Specific Notices for CD3 epsilon Antibody (RIV9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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