CD3 zeta Antibody (BL-336-1B2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76399

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76399
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Porcine (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-336-1B2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 150-164 (C-Term)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 zeta Antibody (BL-336-1B2)

CD3 zeta Antibody (BL-336-1B2)

Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] -

Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.
Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399]

Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399]

Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Zrecombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399]

Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399]

Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
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Applications for CD3 zeta Antibody (BL-336-1B2)

Application
Recommended Usage

Flow Cytometry

0.5 ul per 1 - 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD3 zeta/CD247

T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (BL-336-1B2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta Antibody (BL-336-1B2)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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