CD3 zeta [p Tyr153] Antibody (EM-17)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62192

Novus Biologicals, part of Bio-Techne
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NBP2-62192
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EM-17

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Summary for CD3 zeta [p Tyr153] Antibody (EM-17)

Immunogen

A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH

Modification

p Tyr153

Specificity

The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD3 zeta [p Tyr153] Antibody (EM-17)

Western Blot: CD3 zeta [p Tyr153] Antibody (EM-17) [NBP2-62192] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.

Applications for CD3 zeta [p Tyr153] Antibody (EM-17)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta [p Tyr153] Antibody (EM-17)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta [p Tyr153] Antibody (EM-17)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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