CD3 zeta [p Tyr72] Antibody (EM-26)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62193

Novus Biologicals, part of Bio-Techne
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NBP2-62193
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # EM-26

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Specifications

Immunogen

A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH

Modification

p Tyr72

Specificity

The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CD3 zeta [p Tyr72] Antibody (EM-26)

Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.
Western Blot: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.
CD3 zeta [p Tyr72] Antibody (EM-26) Flow Cytometry: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193]

Flow Cytometry: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193]

Anti-Hu CD3 zeta (p72) purified antibody (clone EM-26) specificity verification by flow cytometry

Anti-Hu CD3 zeta (pY72) purified antibody (concentration in sample 1 ug/ml, red-filled histogram) binds specifically to intracellular CD3 zeta (pY72) in pervanadate treated and then methanol permeabilized Jurkat cells (upper panel), but not to untreated methanol permeabilized Jurkat cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 1 ug/ml, black-dashed histogram).

Applications for CD3 zeta [p Tyr72] Antibody (EM-26)

Application
Recommended Usage

Flow (Intracellular)

1-9 ug/ml

Western Blot

2-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta [p Tyr72] Antibody (EM-26)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 zeta [p Tyr72] Antibody (EM-26)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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