CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37709
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Flow (Intracellular), Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # EM-55
Format
BSA Free
Concentration
1 mg/ml
Product Summary for CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free
Immunogen
BP1 (pTyr111/123) - KLH.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Modification
p Tyr111
Specificity
Antibody recognizes phosphorylated tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free
Western Blot: CD3 zeta [p Tyr111] Antibody (EM-55) [NBP2-37709] - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).
Western Blot: CD3 zeta [p tyr 111] Antibody (EM-55) [NBP2-37709] - Analysis in lysates of HEK293T/17 cells transiently transfected with expression vector harboring gene for mCD3zeta. Lane 1) Unstimulated cells, Lane 2) Pervanadate-stimulated cells
![CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free Flow Cytometry: CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free [NBP2-37709]](https://resources.bio-techne.com/images/products/nbp2-37709_mouse-monoclonal-cd3-zeta-p-tyr111-antibody-em-55-13120241449501.jpg "Flow Cytometry: CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free [NBP2-37709]")
Flow Cytometry: CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free [NBP2-37709]
Anti-Hu CD3 zeta (p111) purified antibody (clone EM-55) specificity verification by flow cytometryAnti-Hu CD3 zeta (pY111) purified antibody (concentration in sample 1 ug/ml, red-filled histogram) binds specifically to intracellular CD3 zeta (pY111) in pervanadate treated and then methanol permeabilized Jurkat cells (upper panel), but not to untreated methanol permeabilized Jurkat cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 1 ug/ml, black-dashed histogram).
Applications for CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
2-6 ug/ml
Flow Cytometry
1-4 ug/ml
Western Blot
1 ug/ml
Application Notes
Western blotting: Recommended dilution: 1 ug/ml; non-reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD3 zeta/CD247
Long Name
T-cell Receptor zeta
Alternate Names
CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain
Gene Symbol
CD247
Additional CD3 zeta/CD247 Products
Product Documents for CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free
Product Specific Notices for CD3 zeta [p Tyr111] Antibody (EM-55) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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