CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11991
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free [NBP3-11991]](https://resources.bio-techne.com/images/products/CD30-Ligand-TNFSF8-Antibody-RM153-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11991-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free [NBP3-11991]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # RM153
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD30 Ligand/TNFSF8 and fusing the splenocytes with P3U1 myeloma cells.
Specificity
This antibody is specific for murine CD30 Ligand/TNFSF8, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD30 Ligand/TNFSF8 plays an important role in T cell activation/costimulation.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free [NBP3-11991]
Immunocytochemistry/Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric [NBP3-11991] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RG9-35 (NBP3-11991) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11991, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional Assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD30 Ligand/TNFSF8
Alternate Names
CD153, CD30L, TNFSF8
Gene Symbol
TNFSF8
Additional CD30 Ligand/TNFSF8 Products
Product Documents for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free
Product Specific Notices for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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