CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52682

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52682-0.025mg
NBP2-52682-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Cited:

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # Ki-3

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD30/TNFRSF8 Antibodies »

Product Specifications

Immunogen

Human CD30.

Specificity

The antibody binds to CD30.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free

Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] -

Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.
Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free [NBP2-52682]

Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free [NBP2-52682]

Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (NBP2-52682, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Applications for CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. The antibody binds specifically to CD30/TNFRSF8 a cell membrane protein which belongs to the tumour necrosis factor receptor family. Incubation of CD30/TNFRSF8 positive L540 cells with Ki-3 antibody weakly enhances release of soluble CD30/TNFRSF8 (in contrast to Ki-4, NBP2-52714). CD30/TNFRSF8 is expressed on activated lymphocytes, and binding by its ligands TRAF2 and TRAF5 results in the activation of NF-kappaB, thereby regulating gene expression. CD30/TNFRSF8 is also associated with Hodgkin Lymphoma, and its expression acts as a tumour marker. Targeting the CD30/TNFRSF8 antigen with specific monoclonal antibodies is a possible therapy for Hodgkin's lymphoma.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD30/TNFRSF8

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Alternate Names

CD30, TNFRSF8

Gene Symbol

TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD30/TNFRSF8 Antibody (Ki-3) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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