CD30/TNFRSF8 Antibody (SPM609)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44562
![Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (SPM609) [NBP2-44562]](https://resources.bio-techne.com/images/products/CD30-TNFRSF8-Antibody-SPM609-Immunohistochemistry-Paraffin-NBP2-44562-img0001.jpg "Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (SPM609) [NBP2-44562]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM609
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human CD30/TNFRSF8 protein (Uniprot: P28908)
Localization
Cell surface
Specificity
Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkins disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This monoclonal antibody distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
Marker
Hodgkin & Reed-Sternberg Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47907)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD30/TNFRSF8 Antibody (SPM609)
Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (SPM609) [NBP2-44562]
Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (SPM609) [NBP2-44562] - Human Hodgkin's Lymphoma stained with CD30 Monoclonal Antibody (SPM609).Applications for CD30/TNFRSF8 Antibody (SPM609)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD30/TNFRSF8
Additional CD30/TNFRSF8 Products
Product Documents for CD30/TNFRSF8 Antibody (SPM609)
Product Specific Notices for CD30/TNFRSF8 Antibody (SPM609)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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