Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Porcine

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LCI-4

Concentration

1.0 mg/ml

View all CD31/PECAM-1 Antibodies »

Product Specifications

Immunogen

Porcine CD31/human IgGFc fusion protein

Reactivity Notes

Predicted cross-reactivities: Human

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (LCI-4)

Flow Cytometry: CD31/PECAM-1 Antibody (LCI-4) - BSA Free [NB100-65336]

Flow Cytometry: CD31/PECAM-1 Antibody (LCI-4) - BSA Free [NB100-65336]

Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.

Applications for CD31/PECAM-1 Antibody (LCI-4)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Clone LCI4 has been shown to stimulate tyrosine phosphorylation of CD31 on endothelial cells. For Flow Cytometry use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood. This antibody does not work in Western Blotting.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 396941 (Porcine)

Gene Symbol

PECAM1

UniProt

Q95242

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (LCI-4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD31/PECAM-1 Antibody (LCI-4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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