CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80639

Novus Biologicals, part of Bio-Techne
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NBP2-80639
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Mouse

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # MEC 7.46

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD31/PECAM-1 Antibodies »

Product Specifications

Immunogen

This CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free was developed against mouse endothelial cell line T-end.

Localization

Membrane; Single-pass type I membrane protein. Cell junction.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody (MEC 7.46) at 5.0 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639]

Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639]

Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the A
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free [NBP2-80639] - A surface stain was performed on MS-1 Cells with CD31/PECAM-1 Antibody (MEC 7.46) NB100-1642 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems). Image from the standard format of this antibody.
View 3 more images

Applications for CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (PMID 25525387)

Immunoprecipitation

1:10-1:500

In vivo assay

reported in scientific literature (PMID 10544206)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD31/PECAM-1 Antibody (MEC 7.46) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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