CD34 Antibody (QBEnd/10 + HPCA1/763)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44567

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda/IgG1 Lambda Clone # QBEnd/10 + HPCA1/763

Concentration

0.2 mg/ml

View all CD34 Antibodies »

Product Specifications

Immunogen

Detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10); Recombinant full-length human CD34 protein (CD34/763) (Uniprot: P28906)

Localization

Cell surface

Specificity

This monoclonal antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but with a lower specificity.

Marker

Hematopoietic Stem Cell & Endothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda/IgG1 Lambda

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-47909)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for CD34 Antibody (QBEnd/10 + HPCA1/763)

Immunohistochemistry: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567]

Immunohistochemistry: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567]

Immunohistochemistry: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Representative images of RCC patient biopsies immunostained for VEGFR2, CD34, and NRP1 and counterstained using Hoechst33342. Left column shows examples of NRP1 expressed by endothelial cells; middle column shows a tumor sample with low NRP1 expression. Right column shows NRP1 positive perivascular tumor cells. Blood vessels were stained for CD34 and nuclei counterstained using Hoechst33342. Left column shows cis complexes, localized within the endothelium. Middle column shows a tumor lacking VEGFR2/NRP1 complexes. Right column shows trans complexes, located adjacent to the endothelium (white arrowheads). Image collected and cropped by Citeab from the following publication (Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. J Pathol (2020) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763)
Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Human Colon Carcinoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763)
View 2 more images

Applications for CD34 Antibody (QBEnd/10 + HPCA1/763)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
Use in Proximity Ligation Assay reported in scientific literature (PMID: 31880322).
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD34

CD34, also known as Cluster of Differentiation 34, is a transmembrane phosphoglycoprotein that is primarily known for being a surface marker for hematopoietic stem cells (HSCs) and progenitor cells (1,2). The CD34 protein consists of a heavily glycosylated extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with PDZ binding motifs (1,3). Human CD34 has two protein isoforms consisting of 385 amino acids (aa) for the canonical isoform and 328 aa for isoform 2 (3,4). The canonical CD34 isoform has calculated theoretical molecular weight of 40 kDa and an observed molecular weight of ~115 kDa (1,3,4). L-Selectin (CD62L) is the most common ligand for CD34, but it has also been shown to bind CrkL (1,3).

CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).

References

1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13

3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571

4. Uniprot (P28906)

5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02

6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027

Alternate Names

CD34, HPCA1

Gene Symbol

CD34

UniProt

P28906

Additional CD34 Products

Product Documents for CD34 Antibody (QBEnd/10 + HPCA1/763)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD34 Antibody (QBEnd/10 + HPCA1/763)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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